@Avery_USA It is a step of my experiment. I centrifuged the cells and they became a pellet at the bottom of the tube. After discarding the supernatant I add some lysis buffer to resuspend the cells. The volume of the lysis buffer is supposed to three times of that of the pellet, e.g. if the volume of the pellet is around 10 ul, I will add 30 ul lysis buffer. How would you say that? Thank you.